control empty mock pegfp n1 flag vector Search Results


empty pegfp n1 vector mock transfection  (New England Biolabs)
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New England Biolabs empty pegfp n1 vector mock transfection
Empty Pegfp N1 Vector Mock Transfection, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pδegfp-n1-vector  (Becton Dickinson)
90
Becton Dickinson pδegfp-n1-vector
Pδegfp N1 Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pegfp n1 vector control dna  (Thermo Fisher)
99
Thermo Fisher pegfp n1 vector control dna
Pegfp N1 Vector Control Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector pegfp n1  (Addgene inc)
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Addgene inc vector pegfp n1
Vector Pegfp N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pegfp-n1  (Becton Dickinson)
90
Becton Dickinson pegfp-n1
Pegfp N1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pegfp n1 flag vector  (Addgene inc)
93
Addgene inc pegfp n1 flag vector
NIPBL is relevant to the growth of ESCC cells. A, Western blotting analysis of NIPBL expression in ESCC cell lines. GAPDH is shown as loading control. Normal esophageal squamous epithelial tissue from 2 patients, <t>N1</t> and N2, were used as the control. B, Western blotting analysis of NIPBL expression in COLO-680N cells transfected with the NIPBL overexpressing vector. GAPDH is shown as loading control. C, Relative cell proliferation of COLO-680N with NIPBL overexpression was determined by the MTS assay. Cells were transfected with <t>pEGFP-N1-FLAG</t> vector or NIPBL recombinant vector respectively, and the relative cell proliferation was determined by MTS assay after transfection for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001). NIPBL expression in EC9706 cells transfected with NIPBL siRNA was determined by quantitative real-time PCR (D) and western blotting analysis (E). siRNA 1 and siRNA 2 are 2 different NIPBL siRNAs, whereas control is a non-targeting scrambled control siRNA. F, Relative cell proliferation in Eca-109 and EC9706 cells with NIPBL depletion was determined by MTS assay after transfection with NIPBL siRNA for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001).
Pegfp N1 Flag Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pegfp n1 flag vector - by Bioz Stars, 2026-06
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pegfp-n1 vector  (Promega)
90
Promega pegfp-n1 vector
NIPBL is relevant to the growth of ESCC cells. A, Western blotting analysis of NIPBL expression in ESCC cell lines. GAPDH is shown as loading control. Normal esophageal squamous epithelial tissue from 2 patients, <t>N1</t> and N2, were used as the control. B, Western blotting analysis of NIPBL expression in COLO-680N cells transfected with the NIPBL overexpressing vector. GAPDH is shown as loading control. C, Relative cell proliferation of COLO-680N with NIPBL overexpression was determined by the MTS assay. Cells were transfected with <t>pEGFP-N1-FLAG</t> vector or NIPBL recombinant vector respectively, and the relative cell proliferation was determined by MTS assay after transfection for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001). NIPBL expression in EC9706 cells transfected with NIPBL siRNA was determined by quantitative real-time PCR (D) and western blotting analysis (E). siRNA 1 and siRNA 2 are 2 different NIPBL siRNAs, whereas control is a non-targeting scrambled control siRNA. F, Relative cell proliferation in Eca-109 and EC9706 cells with NIPBL depletion was determined by MTS assay after transfection with NIPBL siRNA for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001).
Pegfp N1 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pegfp n1 ub g76v gfp plasmid  (Addgene inc)
92
Addgene inc pegfp n1 ub g76v gfp plasmid
NIPBL is relevant to the growth of ESCC cells. A, Western blotting analysis of NIPBL expression in ESCC cell lines. GAPDH is shown as loading control. Normal esophageal squamous epithelial tissue from 2 patients, <t>N1</t> and N2, were used as the control. B, Western blotting analysis of NIPBL expression in COLO-680N cells transfected with the NIPBL overexpressing vector. GAPDH is shown as loading control. C, Relative cell proliferation of COLO-680N with NIPBL overexpression was determined by the MTS assay. Cells were transfected with <t>pEGFP-N1-FLAG</t> vector or NIPBL recombinant vector respectively, and the relative cell proliferation was determined by MTS assay after transfection for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001). NIPBL expression in EC9706 cells transfected with NIPBL siRNA was determined by quantitative real-time PCR (D) and western blotting analysis (E). siRNA 1 and siRNA 2 are 2 different NIPBL siRNAs, whereas control is a non-targeting scrambled control siRNA. F, Relative cell proliferation in Eca-109 and EC9706 cells with NIPBL depletion was determined by MTS assay after transfection with NIPBL siRNA for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001).
Pegfp N1 Ub G76v Gfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid 38132  (Addgene inc)
93
Addgene inc plasmid 38132
NIPBL is relevant to the growth of ESCC cells. A, Western blotting analysis of NIPBL expression in ESCC cell lines. GAPDH is shown as loading control. Normal esophageal squamous epithelial tissue from 2 patients, <t>N1</t> and N2, were used as the control. B, Western blotting analysis of NIPBL expression in COLO-680N cells transfected with the NIPBL overexpressing vector. GAPDH is shown as loading control. C, Relative cell proliferation of COLO-680N with NIPBL overexpression was determined by the MTS assay. Cells were transfected with <t>pEGFP-N1-FLAG</t> vector or NIPBL recombinant vector respectively, and the relative cell proliferation was determined by MTS assay after transfection for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001). NIPBL expression in EC9706 cells transfected with NIPBL siRNA was determined by quantitative real-time PCR (D) and western blotting analysis (E). siRNA 1 and siRNA 2 are 2 different NIPBL siRNAs, whereas control is a non-targeting scrambled control siRNA. F, Relative cell proliferation in Eca-109 and EC9706 cells with NIPBL depletion was determined by MTS assay after transfection with NIPBL siRNA for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001).
Plasmid 38132, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pegfp n1 vector  (Addgene inc)
93
Addgene inc pegfp n1 vector
NIPBL is relevant to the growth of ESCC cells. A, Western blotting analysis of NIPBL expression in ESCC cell lines. GAPDH is shown as loading control. Normal esophageal squamous epithelial tissue from 2 patients, <t>N1</t> and N2, were used as the control. B, Western blotting analysis of NIPBL expression in COLO-680N cells transfected with the NIPBL overexpressing vector. GAPDH is shown as loading control. C, Relative cell proliferation of COLO-680N with NIPBL overexpression was determined by the MTS assay. Cells were transfected with <t>pEGFP-N1-FLAG</t> vector or NIPBL recombinant vector respectively, and the relative cell proliferation was determined by MTS assay after transfection for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001). NIPBL expression in EC9706 cells transfected with NIPBL siRNA was determined by quantitative real-time PCR (D) and western blotting analysis (E). siRNA 1 and siRNA 2 are 2 different NIPBL siRNAs, whereas control is a non-targeting scrambled control siRNA. F, Relative cell proliferation in Eca-109 and EC9706 cells with NIPBL depletion was determined by MTS assay after transfection with NIPBL siRNA for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001).
Pegfp N1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pegfp n1 vector  (New England Biolabs)
99
New England Biolabs pegfp n1 vector
NIPBL is relevant to the growth of ESCC cells. A, Western blotting analysis of NIPBL expression in ESCC cell lines. GAPDH is shown as loading control. Normal esophageal squamous epithelial tissue from 2 patients, <t>N1</t> and N2, were used as the control. B, Western blotting analysis of NIPBL expression in COLO-680N cells transfected with the NIPBL overexpressing vector. GAPDH is shown as loading control. C, Relative cell proliferation of COLO-680N with NIPBL overexpression was determined by the MTS assay. Cells were transfected with <t>pEGFP-N1-FLAG</t> vector or NIPBL recombinant vector respectively, and the relative cell proliferation was determined by MTS assay after transfection for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001). NIPBL expression in EC9706 cells transfected with NIPBL siRNA was determined by quantitative real-time PCR (D) and western blotting analysis (E). siRNA 1 and siRNA 2 are 2 different NIPBL siRNAs, whereas control is a non-targeting scrambled control siRNA. F, Relative cell proliferation in Eca-109 and EC9706 cells with NIPBL depletion was determined by MTS assay after transfection with NIPBL siRNA for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001).
Pegfp N1 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


NIPBL is relevant to the growth of ESCC cells. A, Western blotting analysis of NIPBL expression in ESCC cell lines. GAPDH is shown as loading control. Normal esophageal squamous epithelial tissue from 2 patients, N1 and N2, were used as the control. B, Western blotting analysis of NIPBL expression in COLO-680N cells transfected with the NIPBL overexpressing vector. GAPDH is shown as loading control. C, Relative cell proliferation of COLO-680N with NIPBL overexpression was determined by the MTS assay. Cells were transfected with pEGFP-N1-FLAG vector or NIPBL recombinant vector respectively, and the relative cell proliferation was determined by MTS assay after transfection for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001). NIPBL expression in EC9706 cells transfected with NIPBL siRNA was determined by quantitative real-time PCR (D) and western blotting analysis (E). siRNA 1 and siRNA 2 are 2 different NIPBL siRNAs, whereas control is a non-targeting scrambled control siRNA. F, Relative cell proliferation in Eca-109 and EC9706 cells with NIPBL depletion was determined by MTS assay after transfection with NIPBL siRNA for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001).

Journal: Technology in Cancer Research & Treatment

Article Title: Nipped-B-like Protein Sensitizes Esophageal Squamous Cell Carcinoma Cells to Cisplatin via Upregulation of PUMA

doi: 10.1177/1533033820960726

Figure Lengend Snippet: NIPBL is relevant to the growth of ESCC cells. A, Western blotting analysis of NIPBL expression in ESCC cell lines. GAPDH is shown as loading control. Normal esophageal squamous epithelial tissue from 2 patients, N1 and N2, were used as the control. B, Western blotting analysis of NIPBL expression in COLO-680N cells transfected with the NIPBL overexpressing vector. GAPDH is shown as loading control. C, Relative cell proliferation of COLO-680N with NIPBL overexpression was determined by the MTS assay. Cells were transfected with pEGFP-N1-FLAG vector or NIPBL recombinant vector respectively, and the relative cell proliferation was determined by MTS assay after transfection for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001). NIPBL expression in EC9706 cells transfected with NIPBL siRNA was determined by quantitative real-time PCR (D) and western blotting analysis (E). siRNA 1 and siRNA 2 are 2 different NIPBL siRNAs, whereas control is a non-targeting scrambled control siRNA. F, Relative cell proliferation in Eca-109 and EC9706 cells with NIPBL depletion was determined by MTS assay after transfection with NIPBL siRNA for 72 h. All experiments were repeated thrice and the representative results are shown. The statistical significance is p < 0.001 (Student’s t -test, *** represents p < 0.001).

Article Snippet: NIPBL ORF (1-8,094 bp) was cloned into the pEGFP-N1-FLAG vector (Addgene, Watertown, Massachusetts, USA).

Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation, Over Expression, MTS Assay, Recombinant, Real-time Polymerase Chain Reaction